비스포스포네이트가 인간 치수 줄기세포의 조골세포로의 분화와 광물화에 미치는 영향
Effect of Bisphosphonates on Osteoblast Differentiation and Mineralization of Human Dental Pulp Stem Cell
오종식, 유미현, 김형준, 신상훈, 이재열,
소속 상세정보
오종식 ( Oh Jong-Shik ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
유미현 ( Ryu Mi-Heon ) - 부산대학교 치의학전문대학원 구강병리학교실
김형준 ( Kim Hyung-Joon ) - 부산대학교 치의학전문대학원 구강생리학교실
신상훈 ( Shin Sang-Hun ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
이재열 ( Lee Jae-Yeol ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
Abstract
In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.
키워드
Bisphosphonate; Dental pulp stem cell; Osteoblast differentiation
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